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Product Name
KLHL18 antibody
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Description
KLHL18 Rabbit Polyclonal antibody. Positive IHC detected in human colon. Positive IF detected in Hela. Positive WB detected in HepG2 cells, HEK-293 cells, COLO 320 cells, HeLa cells, HL-60 cells, mouse brain tissue. Observed molecular weight by Western-blot: 63kd,56kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
KLHL18 antibody; Kelch like protein 18 antibody; FLJ61265 antibody; kelch like 18 (Drosophila) antibody; KIAA0795 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KLHL18 recombinant protein (Accession Number: BC032620). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112095(KLHL18 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon using Catalog No:112095(KLHL18 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:112095(KLHL18 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using KLHL18 antibody Catalog No:112095 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
KLHL18 represents kelch-like 18 (Drosophila)
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References
- Moghe S, Jiang F, Miura Y, Cerny RL, Tsai MY, Furukawa M. The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A. Biology open. 1(2):82-91. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"