KLHL12 antibody

Cat.#: 112089

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Product Information

  • Product Name
    KLHL12 antibody
  • Documents
  • Description
    KLHL12 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human testis tissue. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 53-56 kDa,63-67 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C3IP1 antibody; CUL3 interacting protein 1 antibody; DKIR antibody; FLJ27152 antibody; kelch like 12 (Drosophila) antibody; Kelch like protein 12 antibody; KLHL12 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of KLHL12 recombinant protein (Accession Number: NM_021633). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112089(KLHL12 antibody) at dilution of 1:1000

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112089(KLHL12 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of PC-3 cells using Catalog No:112089(KLHL12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of PC-3 cells using Catalog No:112089(KLHL12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    KLHL12 represents kelch-like 12 (Drosophila)
  • References
    • Butkinaree C, Guo L, Ramkhelawon B. A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report. Arteriosclerosis, thrombosis, and vascular biology. 34(2):251-4. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"