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Product Name
KLF5 antibody
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Description
KLF5 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A549 cells, HT-1080 cells, human testis tissue, mouse colon tissue, mouse small intestine tissue, PC-3 cells. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BTE binding protein 2 antibody; BTEB2 antibody; CKLF antibody; Colon krueppel like factor antibody; GC box binding protein 2 antibody; IKLF antibody; KLF5 antibody; Krueppel like factor 5 antibody; Transcription factor BTEB2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KLF5 recombinant protein (Accession Number: NM_001730). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112082(KLF5 Antibody) at dilution of 1:1000
IP Result of anti-KLF5 (IP:Catalog No:112082, 4ug; Detection:Catalog No:112082 1:500) with HeLa cells lysate 2800ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:112082(KLF5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.
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References
- Ji S, Qin Y, Shi S. ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. Cell research. 25(5):561-73. 2015.
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