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Product Name
KISS1 antibody
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Description
KISS1 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, human placenta tissue, mouse brain tissue. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 16-20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KiSS 1 antibody; KiSS 1 metastasis suppressor antibody; KISS1 antibody; Kisspeptin 1 antibody; Metastasis suppressor KiSS 1 antibody; metastin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KISS1 recombinant protein (Accession Number: NM_002256). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112071(KISS1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112071(KISS1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112071(KISS1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:112071(KISS1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).
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