Kininogen 1 antibody

Cat.#: 112057

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Product Information

  • Product Name
    Kininogen 1 antibody
  • Documents
  • Description
    Kininogen 1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A2780 cells, COLO 320 cells, MCF7 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue. Observed molecular weight by Western-blot: 60 kDa or 47 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    BDK antibody; Fitzgerald factor antibody; HMWK antibody; Ile Ser Bradykinin Bradykinin antibody; Kallidin I Lysyl bradykinin antibody; kininogen 1 antibody; KNG antibody; KNG1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Kininogen 1 recombinant protein (Accession Number: NM_000893). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112057(KNG1 antibody) at dilution of 1:300

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112057(KNG1 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112057(KNG1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112057(KNG1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112057(KNG1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112057(KNG1 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using KNG1 antibody Catalog No:112057 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using KNG1 antibody Catalog No:112057 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"