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Product Name
KIF3A antibody
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Description
KIF3A Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 80kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIF3 antibody; KIF3A antibody; kinesin family member 3A antibody; Kinesin like protein KIF3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KIF3A recombinant protein (Accession Number: NM_001300792). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
IP Result of anti-KIF3A (IP:Catalog No:112013, 4ug; Detection:Catalog No:112013 1:500) with mouse brain tissue lysate 8000ug.
WB and IP result of the antibdoy anti-KIF3A (Catalog No:112013) in mouse cell from Corbit, Kevin.
human cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:112013(KIF3A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112013(KIF3A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112013(KIF3A antibody) at dilution of 1:100 (under 40x lens)
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Background
KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)
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References
- Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. The EMBO journal. 32(4):597-607. 2013.
- Guadiana SM, Semple-Rowland S, Daroszewski D. Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(6):2626-38. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"