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Product Name
KEAP1 antibody
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Description
KEAP1 Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in HepG2 cells, HEK-293 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue. Positive IHC detected in human lung cancer tissue, human breast cancer tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 55-70 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cytosolic inhibitor of Nrf2 antibody; INrf2 antibody; KEAP1 antibody; Kelch like protein 19 antibody; KIAA0132 antibody; KLHL19 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KEAP1 recombinant protein (Accession Number: XM_011528452). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of KEAP1 antibody (Catalog No:112032, 1:2000) with si-control and si-KEAP1 transfected HEK293 cell.
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112032(KEAP1 antibody) at dilution of 1:2000
IP Result of anti-KEAP1 (IP:Catalog No:112032, 5ug; Detection:Catalog No:112032 1:600) with mouse skeletal muscle tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112032(KEAP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112032(KEAP1 antibody) at dilution of 1:100 (under 40x lens)
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Background
KEAP1, also named as INRF2, KIAA0132 and KLHL19, is part of a multiprotein complex that contains the CUL3-Roc1 ubiquitin ligase, which can ubiquitinate the N-terminal domain of NRF2[PMID: 20173742]. Two molecules of KEAP1 bind to two distinct sites in the N-terminal region of NRF2, the ETGE and DLG sites, which affect the KEAP1-NRF2 interaction and/or its physiological consequences[PMID: 22215675]. KEAP1 retains NFE2L2/NRF2 in the cytosol. It functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1[PMID: 20427290]. It also retains BPTF in the cytosol. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human KEAP1. Read more about this antibody on the blog:http://blog.ptglab.com/index.php/nsclc-research-anti-keap1-antibody-helps-uncover-targets-biomarker-potential/
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References
- Lee DF, Kuo HP, Liu M. KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta. Molecular cell. 36(1):131-40. 2009.
- Awuh JA, Haug M, Mildenberger J. Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages. Proceedings of the National Academy of Sciences of the United States of America. 112(31):E4272-80. 2015.
- Park JS, Kang DH, Bae SH. p62 prevents carbonyl cyanide m-chlorophenyl hydrazine (CCCP)-induced apoptotic cell death by activating Nrf2. Biochemical and biophysical research communications. 464(4):1139-44. 2015.
- Park JS, Kang DH, Lee da H, Bae SH. Fenofibrate activates Nrf2 through p62-dependent Keap1 degradation. Biochemical and biophysical research communications. 465(3):542-7. 2015.
- Park JS, Kang DH, Lee da H, Bae SH. Concerted action of p62 and Nrf2 protects cells from palmitic acid-induced lipotoxicity. Biochemical and biophysical research communications. 466(1):131-7. 2015.
- Park JS, Kang DH, Lee da H, Bae SH. PF-4708671, a specific inhibitor of p70 ribosomal S6 kinase 1, activates Nrf2 by promoting p62-dependent autophagic degradation of Keap1. Biochemical and biophysical research communications. 466(3):499-504. 2015.
- Eino A, Kageyama S, Uemura T. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. Journal of cell science. 128(23):4453-61. 2015.
- Kageyama S, Sou YS, Uemura T. Proteasome dysfunction activates autophagy and the Keap1-Nrf2 pathway. The Journal of biological chemistry. 289(36):24944-55. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"