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Product Name
KCNJ15 antibody
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Description
KCNJ15 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells. Positive IP detected in HepG2 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
IRKK antibody; KCNJ14 antibody; KCNJ15 antibody; KIR1.3 antibody; KIR4.2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KCNJ15 recombinant protein (Accession Number: XM_011529560). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111969(KCNJ15 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:111969(KCNJ15 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111969(KCNJ15 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-KCNJ15 (IP:Catalog No:111969, 4ug; Detection:Catalog No:111969 1:500) with HepG2 cells lysate 1000ug.
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Background
KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)
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References
- Nakajima K, Zhu K, Sun YH. KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis. Nature communications. 6:8532. 2015.
- Kobayashi A, Donaldson DS, Kanaya T. Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations. DNA research : an international journal for rapid publication of reports on genes and genomes. 19(5):407-22. 2012.
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