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Product Name
KAT5 antibody
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Description
KAT5 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive WB detected in PC-3 cells, HeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
60 kDa Tat interactive protein antibody; cPLA(2) interacting protein antibody; cPLA2 antibody; ESA1 antibody; Histone acetyltransferase KAT5 antibody; HIV 1 Tat interactive protein antibody; HTATIP antibody; HTATIP1 antibody; K(lysine) acetyltransferase 5 antibody; KAT5 antibody; Lysine acetyltransferase 5 antibody; PLIP antibody; TIP antibody; TIP60 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KAT5 recombinant protein (Accession Number: NM_182709). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111913(KAT5 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111913(KAT5 antibody) at dilution of 1:100 (under 10x lens)
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Background
KAT5 belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.
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References
- Obri A, Ouararhni K, Papin C. ANP32E is a histone chaperone that removes H2A.Z from chromatin. Nature. 505(7485):648-53. 2014.
- Yu H, Diao H, Wang C. Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Molecular & cellular proteomics : MCP. 14(4):1009-23. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"