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Product Name
JUNB antibody
- Documents
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Description
JUNB Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in MCF7 cells, C6 cells, HeLa cells, HT-1080 cells. Positive IHC detected in human gliomas tissue, human ovary tissue, human testis tissue. Positive IF detected in MCF-7 cells, HeLa cells. Observed molecular weight by Western-blot: 42 kDa, 43 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 1 antibody; jun B proto oncogene antibody; JUNB antibody; Transcription factor jun B antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of JUNB recombinant protein (Accession Number: NM_002229). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111900(JUNB antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:111900(JUNB antibody) at dilution of 1:50 (under 25x lens)
IP Result of anti-JUNB (IP:Catalog No:111900, 4ug; Detection:Catalog No:111900 1:500) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:111900(JUNB Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"