-
Product Name
JUN antibody
- Documents
-
Description
JUN Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 39 kDa
-
Tested applications
ELISA, WB, IF, IHC, IP
-
Species reactivity
Human, hamster, Mouse; other species not tested.
-
Alternative names
Activator protein 1 antibody; AP 1 antibody; AP1 antibody; c Jun antibody; JUN antibody; jun oncogene antibody; P39 antibody; Proto oncogene c Jun antibody; Transcription factor AP 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of JUN recombinant protein (Accession Number: NM_002228). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111899(JUN Antibody) at dilution of 1:1000
IP Result of anti-JUN (IP:Catalog No:111899, 4ug; Detection:Catalog No:111899 1:1000) with HeLa cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:111899(JUN Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:111899(JUN Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:111899(JUN Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
-
Background
JUN also named as jun oncogene or Activator protein 1 is 331 amino acid protein, which contains 1 basic leucine zipper domain and belongs to the bZIP family. As a transcription factor, JUN recognizes and binds to the enhancer heptamer motif and invovles in the regulation of steroidogenic gene expression upon cAMP signaling pathway stimulation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"