JAML antibody

Cat.#: 111883

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Product Information

  • Product Name
    JAML antibody
  • Documents
  • Description
    JAML Rabbit Polyclonal antibody. Positive IHC detected in human spleen tissue. Positive IF detected in Hela cells. Positive FC detected in HeLa cells. Positive WB detected in HL-60 cells, Jurkat cells, L02 cells. Observed molecular weight by Western-blot: 30kd,44kd,50-60kd
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    AMICA antibody; AMICA1 antibody; CREA7 1 antibody; CREA7 4 antibody; FLJ37080 antibody; Gm638 antibody; JAML antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of JAML recombinant protein (Accession Number: NM_001286571). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:111883(AMICA1 antibody) at dilution of 1:800

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:111883(AMICA1 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111883(AMICA1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111883(AMICA1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111883(AMICA1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111883(AMICA1 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using AMICA1 antibody Catalog No:111883 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using AMICA1 antibody Catalog No:111883 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HeLa cells were stained with 0.2ug AMICA1 antibody (Catalog No:111883, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

    1X10^6 HeLa cells were stained with 0.2ug AMICA1 antibody (Catalog No:111883, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

  • Background
    AMICA1 represents adhesion molecule, interacts with CXADR antigen 1

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"