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Product Name
IVD antibody
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Description
IVD Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF-7 cells, human brain tissue, Transfected HEK-293 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 41 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACAD2 antibody; IVD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IVD recombinant protein (Accession Number: NM_002225). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111962(IVD antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:111962(IVD antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111962(IVD antibody) at dilution of 1:50 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111962(IVD Antibody) at dilution of 1:500
IP Result of anti-IVD (IP:Catalog No:111962, 4ug; Detection:Catalog No:111962 1:300) with MCF-7 cells lysate 800ug.
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Background
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.
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References
- Ryu MJ, Lee C, Kim J, Shin HS, Yu MH. Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure. Journal of neurochemistry. 104(5):1260-70. 2008.
- Gu L, Jones AD, Last RL. Broad connections in the Arabidopsis seed metabolic network revealed by metabolite profiling of an amino acid catabolism mutant. The Plant journal : for cell and molecular biology. 61(4):579-90. 2010.
- Horn S, Kirkegaard JS, Hoelper S. Research Resource: A Dual Proteomic Approach Identifies Regulated Islet Proteins During β-Cell Mass Expansion In Vivo. Molecular endocrinology (Baltimore, Md.). 30(1):133-43. 2016.
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