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Product Name
ITPKC antibody
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Description
ITPKC Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human skeletal muscle tissue, human heart tissue, human lymphoma tissue. Positive WB detected in human heart tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 100-105 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
InsP 3 kinase C antibody; IP3 3 kinase C antibody; IP3K C antibody; IP3KC antibody; ITPKC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ITPKC recombinant protein (Accession Number: XM_047439466). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111959(ITPKC antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells, using ITPKC antibody Catalog No:111959 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-ITPKC (IP:Catalog No:111959, 4ug; Detection:Catalog No:111959 1:400) with HepG2 cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:111959(ITPKC Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:111959(ITPKC Antibody) at dilution of 1:50 (under 40x lens)
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Background
ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).
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