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IRF3 antibody

Cat.#: 111835

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Special Price 239.0 USD

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Product Information

  • Product Name
    IRF3 antibody
  • Documents
  • Description
    IRF3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, human brain tissue, mouse brain tissue, rat brain tissue, U-937 cells. Positive IP detected in Jurkat cells. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue. Observed molecular weight by Western-blot: 49-55 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    interferon regulatory factor 3 antibody; IRF 3 antibody; IRF3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IRF3 recombinant protein (Accession Number: XM_006723198). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111835(IRF3 antibody) at dilution of 1:1000

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111835(IRF3 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111835(IRF3 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111835(IRF3 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using IRF3 antibody Catalog No:111835 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using IRF3 antibody Catalog No:111835 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-IRF3 (IP:Catalog No:111835, 3ug; Detection:Catalog No:111835 1:1000) with Jurkat cells lysate 2400ug.

    IP Result of anti-IRF3 (IP:Catalog No:111835, 3ug; Detection:Catalog No:111835 1:1000) with Jurkat cells lysate 2400ug.

    1X10^6 HepG2 cells were stained with .2ug IRF3 antibody (Catalog No:111835, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug IRF3 antibody (Catalog No:111835, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes
  • References
    • Zhu M, Fang T, Li S, Meng K, Guo D. Bipartite Nuclear Localization Signal Controls Nuclear Import and DNA-Binding Activity of IFN Regulatory Factor 3. Journal of immunology (Baltimore, Md. : 1950). 195(1):289-97. 2015.
    • Li L, Qin JJ, Guo S. Attenuation of Cerebral Ischemic Injury in Interferon Regulatory Factor 3 Deficient Rat. Journal of neurochemistry. 2015.
    • Li S, Zhu M, Pan R. The tumor suppressor PTEN has a critical role in antiviral innate immunity. Nature immunology. 17(3):241-9. 2016.
    • Zhu H, Zheng C, Xing J. Varicella-zoster virus immediate-early protein ORF61 abrogates the IRF3-mediated innate immune response through degradation of activated IRF3. Journal of virology. 85(21):11079-89. 2011.
    • Zhou P, Li H, Wang H, Wang LF, Shi Z. Bat severe acute respiratory syndrome-like coronavirus ORF3b homologues display different interferon antagonist activities. The Journal of general virology. 93(Pt 2):275-81. 2012.
    • Zhang Z, Zheng Z, Luo H. Human bocavirus NP1 inhibits IFN-β production by blocking association of IFN regulatory factor 3 with IFNB promoter. Journal of immunology (Baltimore, Md. : 1950). 189(3):1144-53. 2012.
    • Guo R, Li Y, Ning J, Sun D, Lin L, Liu X. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PloS one. 8(4):e62729. 2013.
    • Luo H, Zhang Z, Zheng Z. Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. Journal of immunology (Baltimore, Md. : 1950). 191(2):660-9. 2013.

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