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Product Name
IRF2BP1 antibody
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Description
IRF2BP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue, rat lymph tissue. Positive IP detected in HEK-293 cells. Positive IF detected in Hela cells. Positive IHC detected in human endometrial cancer tissue. Observed molecular weight by Western-blot: 62-72 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434M154 antibody; IRF 2 binding protein 1 antibody; IRF 2BP1 antibody; IRF2BP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IRF2BP1 recombinant protein (Accession Number: NM_015649). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111833(IRF2BP1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:111833(IRF2BP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:111833(IRF2BP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using IRF2BP1 antibody Catalog No:111833 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-IRF2BP1 (IP:Catalog No:111833, 4ug; Detection:Catalog No:111833 1:500) with HEK-293 cells lysate 2000ug.
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Background
IRF2BP1 represents interferon regulatory factor 2 binding protein 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"