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Product Name
IPO4 antibody
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Description
IPO4 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in HeLa cells, human brain tissue, mouse testis tissue. Observed molecular weight by Western-blot: 118kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ23338 antibody; Imp4 antibody; Imp4b antibody; importin 4 antibody; Importin 4b antibody; IPO4 antibody; Ran binding protein 4 antibody; RanBP4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IPO4 recombinant protein (Accession Number: BC003690). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111820(IPO4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111820(IPO4 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using IPO4 antibody Catalog No:111820 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-IPO4 (IP:Catalog No:111820, 5ug; Detection:Catalog No:111820 1:500) with mouse testis tissue lysate 4000ug.
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Background
IPO4 represents importin 4
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References
- Echeverría PC, Bernthaler A, Dupuis P, Mayer B, Picard D. An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. PloS one. 6(10):e26044. 2011.
- Wang J, Sarkar TR, Zhou M. CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage. Proceedings of the National Academy of Sciences of the United States of America. 107(37):16131-6. 2010.
- Humbert MC, Weihbrecht K, Searby CC. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Proceedings of the National Academy of Sciences of the United States of America. 109(48):19691-6. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"