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Product Name
IPO11 antibody
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Description
IPO11 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in human testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 112 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Imp11 antibody; importin 11 antibody; IPO11 antibody; Ran binding protein 11 antibody; RanBP11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IPO11 recombinant protein (Accession Number: NM_016338). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111818(IPO11 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111818(IPO11 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111818(IPO11 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-IPO11 (IP:Catalog No:111818, 4ug; Detection:Catalog No:111818 1:500) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:111818(IPO11 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. Importin-11, is a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.
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