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Product Name
INVS antibody
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Description
INVS Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse skeletal muscle tissue, Raji cells, SH-SY5Y cells. Positive IHC detected in human breast cancer tissue, human hepat℃irrhosis tissue. Positive IF detected in Hela cells, hTERT-RPE1 cells. Observed molecular weight by Western-blot: 110-115 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
INV antibody; inversin antibody; INVS antibody; KIAA0573 antibody; NephrOCystin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of INVS recombinant protein (Accession Number: BC006370). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111814(INVS antibody) at dilution of 1:100
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111814(INVS antibody) at dilution of 1:100 (under 25x lens)
IF result from Dr. Corbit, Kevin. anti-INVS(also named as NPHP2; Catalog No:111814) marks the ‘inversin compartment’ and basal bodies of Human hTERT-RPE1 cells.
Immunofluorescent analysis of Hela cells, using INVS antibody Catalog No:111814 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of left-right axis. INVS may involve in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of N-terminus of human INVS.
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References
- Leh S, Hultström M, Rosenberger C, Iversen BM. Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats. Virchows Archiv : an international journal of pathology. 459(1):99-108. 2011.
- Oud MM, van Bon BW, Bongers EM. Early presentation of cystic kidneys in a family with a homozygous INVS mutation. American journal of medical genetics. Part A. 164A(7):1627-34. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"