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Product Name
Involucrin antibody
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Description
Involucrin Rabbit Polyclonal antibody. Positive WB detected in Raji cells, NIH/3T3 cells. Positive IHC detected in human skin tissue, human oesophagus tissue. Observed molecular weight by Western-blot: 80-90kd or 56-70kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
involucrin antibody; IVL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005547). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:111813(IVL antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:111813(Involucrin Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:111813(Involucrin Antibody) at dilution of 1:50 (under 40x lens)
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Background
IVL, also named as involucrin, belongs to the involucrin family. It is a part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. This antibody is specific to IVL.
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References
- Mu Y, Zhou H, Wu WJ, Hu LC, Chen HB. Dynamic expression of miR-206-3p during mouse skin development is independent of keratinocyte differentiation. Molecular medicine reports. 12(6):8113-20. 2015.
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