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Product Name
INSIG1 antibody
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Description
INSIG1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human kidney tissue. Positive IF detected in MCF-7 cells. Positive WB detected in A549 cells, HepG2 cells, MCF7 cells, SGC-7901 cells. Observed molecular weight by Western-blot: 28-31; 66-70 kDa
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CL 6 antibody; INSIG 1 antibody; INSIG1 antibody; insulin induced gene 1 antibody; Insulin induced gene 1 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of INSIG1 recombinant protein (Accession Number: NM_001346592). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111848(INSIG1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:111848(INSIG1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:111848(INSIG1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
INSIG1 represents insulin induced gene 1
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References
- Dang R, Jiang P, Cai H. Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: involvement of the INSIG/SREBP pathway. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology. 25(8):1239-47. 2015.
- Cai HL, Tan QY, Jiang P. A potential mechanism underlying atypical antipsychotics-induced lipid disturbances. Translational psychiatry. 5:e661. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"