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Product Name
INF2 antibody
- Documents
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Description
INF2 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HeLa cells, mouse brain tissue, NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Positive IHC detected in human kidney tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 180-200 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C14orf151 antibody; C14orf173 antibody; DKFZp762A0214 antibody; FLJ22056 antibody; HBEBP2 binding protein C antibody; INF2 antibody; Inverted formin 2 antibody; pp9484 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of INF2 recombinant protein (Accession Number: BC008756). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:111789(INF2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111789(INF2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111789(INF2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-INF2 (IP:Catalog No:111789, 4ug; Detection:Catalog No:111789 1:500) with NIH/3T3 cells lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:111789(INF2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
INF2 represents inverted formin, FH2 and WH2 domain containing
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References
- Shao X, Li Q, Mogilner A, Bershadsky AD, Shivashankar GV. Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim. Proceedings of the National Academy of Sciences of the United States of America. 112(20):E2595-601. 2015.
- Tamura H, Nakazato H, Kuraoka S, Yoneda K, Takahashi W, Endo F. Reduced INF2 expression in nephrotic syndrome is possibly related to clinical severity of steroid resistance in children. Nephrology (Carlton, Vic.). 2015.
- Skau CT, Plotnikov SV, Doyle AD, Waterman CM. Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly. Proceedings of the National Academy of Sciences of the United States of America. 112(19):E2447-56. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"