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Product Name
IMPDH1 antibody
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Description
IMPDH1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, mouse spleen tissue. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor tissue, human tonsillitis tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 53-60 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
DKFZp781N0678 antibody; IMP dehydrogenase 1 antibody; IMPD antibody; IMPD 1 antibody; IMPD1 antibody; IMPDH 1 antibody; IMPDH I antibody; IMPDH1 antibody; LCA11 antibody; RP10 antibody; sWSS2608 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IMPDH1 recombinant protein (Accession Number: NM_183243). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111784(IMPDH1 antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human ovary tumor slide using Catalog No:111784(IMPDH1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human ovary tumor slide using Catalog No:111784(IMPDH1 Antibody) at dilution of 1:50
IP Result of anti-IMPDH1 (IP:Catalog No:111784, 4ug; Detection:Catalog No:111784 1:2000) with HeLa cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:111784(IMPDH1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has 7 isoforms produced by alternative splicing.
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References
- Gunter JH, Thomas EC, Lengefeld N. Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms. The international journal of biochemistry & cell biology. 40(9):1716-28. 2008.
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