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Product Name
ILK antibody
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Description
ILK Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse heart tissue, human heart tissue, mouse brain tissue, mouse kidney tissue, mouse spleen tissue. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 51-59 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
DKFZp686F1765 antibody; ILK antibody; ILK 1 antibody; ILK 2 antibody; ILK1 antibody; ILK2 antibody; P59 antibody; p59ILK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ILK recombinant protein (Accession Number: NM_001014794). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111752(ILK antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111752(ILK antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-ILK (IP:Catalog No:111752, 4ug; Detection:Catalog No:111752 1:800) with mouse kidney tissue lysate 4000ug.
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Background
Integrin-linked kinase (ILK) is an ankyrin-repeat containing serine-threonine protein kinase capable of interacting with the cytoplasmic domains of integrin β1, β2, and β3 subunits and whose kinase activity is modulated by cell-extracellular matrix interactions(PMID: 9539744). This protein belongs to the protein kinase superfamily. It can be autophosphorylated on serine residues.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"