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Product Name
IL-7 antibody
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Description
IL-7 Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells. Positive FC detected in HepG2 cells. Positive IHC detected in human hepat℃irrhosis tissue, human skin tissue. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
IL 7 antibody; IL7 antibody; IL-7 antibody; interleukin 7 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of IL-7 recombinant protein (Accession Number: NM_000880). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107403(IL7 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107403(IL7 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107403(IL7 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HepG2 cells were stained with .2ug IL7 antibody (Catalog No:107403, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
Interleukin-7 (IL7) is a cytokine mportant for B and T cell development. IL7 plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes. It is also a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"