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Product Name
IKBKG antibody
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Description
IKBKG Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue. Positive WB detected in K-562 cells, HEK-293 cells, mouse brain tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMCBX1 antibody; FIP 3 antibody; FIP3 antibody; Fip3p antibody; I kappa B kinase subunit gamma antibody; IkB kinase subunit gamma antibody; IKBKG antibody; IKK gamma antibody; IKKAP1 antibody; IKKG antibody; IP1 antibody; IP2 antibody; IPD2 antibody; NEMO antibody; NF kappa B essential modifier antibody; NF kappa B essential modulator antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IKBKG recombinant protein (Accession Number: NM_001321396). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111645(IKBKG antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111645(IKBKG antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111645(IKBKG antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:111645(IKBKG Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.
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References
- Jia CH, Li M, Liu J. IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1. Cell death and differentiation. 20(2):248-58. 2013.
- Wang W, Zhou J, Shi J. Human T-cell leukemia virus type 1 Tax-deregulated autophagy pathway and c-FLIP expression contribute to resistance against death receptor-mediated apoptosis. Journal of virology. 88(5):2786-98. 2014.
- Yao Z, Zhang P, Guo H. RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages. Molecular oncology. 9(4):806-17. 2015.
- Zhai Y, Wu B, Li J, Yao XY, Zhu P, Chen ZN. CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts. Journal of molecular medicine (Berlin, Germany). 94(1):71-82. 2016.
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