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Product Name
IGFBP1 antibody
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Description
IGFBP1 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human stomach tissue. Positive WB detected in human placenta tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
AFBP antibody; hIGFBP 1 antibody; IBP 1 antibody; IBP1 antibody; IGF binding protein 1 antibody; IGF BP25 antibody; IGFBP 1 antibody; IGFBP1 antibody; Placental protein 12 antibody; PP12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IGFBP1 recombinant protein (Accession Number: NM_000596). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111683(IGFBP1 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:111683(IGFBP1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:111683(IGFBP1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Insulin-like growth factor (IGF)-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and insulin resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.
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References
- Lai G, Zhang Y, Wang L. Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring approach. Prenatal diagnosis. 34(12):1146-52. 2014.
- Feng T, Qiao G, Feng L. Stathmin is key in reversion of doxorubicin resistance by arsenic trioxide in osteosarcoma cells. Molecular medicine reports. 10(6):2985-92. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"