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Product Name
IFT57 antibody
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Description
IFT57 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human pancreas tissue. Positive WB detected in mouse retina tissue, HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 49kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DERP8 antibody; ESRRBL1 antibody; FLJ10147 antibody; HIP1 interacting protein antibody; HIPPI antibody; IFT57 antibody; MHS4R2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IFT57 recombinant protein (Accession Number: NM_018010). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse retina tissue were subjected to SDS PAGE followed by western blot with Catalog No:111670(IFT57 antibody) at dilution of 1:1000
IP Result of anti-IFT57 (IP:Catalog No:111670, 5ug; Detection:Catalog No:111670 1:800) with HEK-293 cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111670(IFT57 antibody) at dilution of 1:50 (under 10x lens)
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Background
IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)
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References
- Seo S, Zhang Q, Bugge K. A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened. PLoS genetics. 7(11):e1002358. 2011.
- Zhang Q, Seo S, Bugge K, Stone EM, Sheffield VC. BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes. Human molecular genetics. 21(9):1945-53. 2012.
- Zhao H, Zhu L, Zhu Y. The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. Nature cell biology. 15(12):1434-44. 2013.
- Luo N, Conwell MD, Chen X. Primary cilia signaling mediates intraocular pressure sensation. Proceedings of the National Academy of Sciences of the United States of America. 111(35):12871-6. 2014.
- Liew GM, Ye F, Nager AR. The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3. Developmental cell. 31(3):265-78. 2014.
- Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.
- Kessler K, Wunderlich I, Uebe S. DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects. Scientific reports. 5:11649. 2015.
- Siller SS, Burke MC, Li FQ, Takemaru K. Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells. Cell cycle (Georgetown, Tex.). 14(19):3163-72. 2015.
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