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IFT52 antibody

Cat.#: 111669

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Product Information

  • Product Name
    IFT52 antibody
  • Documents
  • Description
    IFT52 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in MDCK cells, human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue, HeLa cells. Observed molecular weight by Western-blot: 50kd
  • Tested applications
    ELISA, IF, WB, IP
  • Species reactivity
    Human,Mouse,Rat, Dog; other species not tested.
  • Alternative names
    C20orf9 antibody; CGI 53 antibody; dJ1028D15.1 antibody; IFT52 antibody; NGD5 antibody; Protein NGD5 homolog antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IFT52 recombinant protein (Accession Number: NM_016004). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    MDCK cells were subjected to SDS PAGE followed by western blot with Catalog No:111669(IFT52 Antibody) at dilution of 1:600

    MDCK cells were subjected to SDS PAGE followed by western blot with Catalog No:111669(IFT52 Antibody) at dilution of 1:600

    Immunofluorescent analysis of Hela cells, using IFT52 antibody Catalog No:111669 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using IFT52 antibody Catalog No:111669 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-IFT52 (IP:Catalog No:111669, 4ug; Detection:Catalog No:111669 1:1000) with mouse testis tissue lysate 4000ug.

    IP Result of anti-IFT52 (IP:Catalog No:111669, 4ug; Detection:Catalog No:111669 1:1000) with mouse testis tissue lysate 4000ug.

  • Background
    IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 50-54kd.
  • References
    • Delaval B, Bright A, Lawson ND, Doxsey S. The cilia protein IFT88 is required for spindle orientation in mitosis. Nature cell biology. 13(4):461-8. 2011.
    • Cevik S, Sanders AA, Van Wijk E. Active transport and diffusion barriers restrict Joubert Syndrome-associated ARL13B/ARL-13 to an Inv-like ciliary membrane subdomain. PLoS genetics. 9(12):e1003977. 2013.
    • Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"