-
Product Name
IFT25 antibody
- Documents
-
Description
IFT25 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive WB detected in HeLa cells, A549 cells, mouse brain tissue. Observed molecular weight by Western-blot: 25 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
C1orf41 antibody; Heat shOCk protein beta 11 antibody; HSPB11 antibody; HSPCO34 antibody; IFT25 antibody; ITF25 antibody; Placental protein 25 antibody; PP25 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of IFT25 recombinant protein (Accession Number: NM_016126). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111666(HSPB11 antibody) at dilution of 1:500
IF result (the base of cilia stain) of anti-HSPB11 (IFT25, Catalog No:111666, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.
-
Background
HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.
-
References
- Raman M, Sergeev M, Garnaas M. Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nature cell biology. 17(10):1356-69. 2015.
- Cheng W, Li M, Jiang Y. Association between small heat shock protein B11 and the prognostic value of MGMT promoter methylation in patients with high-grade glioma. Journal of neurosurgery. 2015.
- Keady BT, Samtani R, Tobita K. IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport. Developmental cell. 22(5):940-51. 2012.
- Eguether T, San Agustin JT, Keady BT. IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment. Developmental cell. 31(3):279-90. 2014.
- Yang N, Li L, Eguether T, Sundberg JP, Pazour GJ, Chen J. Intraflagellar transport 27 is essential for hedgehog signaling but dispensable for ciliogenesis during hair follicle morphogenesis. Development (Cambridge, England). 142(12):2194-202. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"