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IFT140 antibody

Cat.#: 111664

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Product Information

  • Product Name
    IFT140 antibody
  • Documents
  • Description
    IFT140 Rabbit Polyclonal antibody. Positive IP detected in rat testis tissue. Positive WB detected in mouse testis tissue, HepG2 cells, rat testis tissue. Positive IF detected in hTERT-RPE1 cells. Observed molecular weight by Western-blot: 140 kDa
  • Tested applications
    ELISA, IF, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    c305C8.4 antibody; c380F5.1 antibody; DKFZp564L232 antibody; FLJ10306 antibody; FLJ30571 antibody; gs114 antibody; IFT140 antibody; KIAA0590 antibody; WDTC2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IFT140 recombinant protein (Accession Number: NM_014714). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:1000

    IF: 1:20-1:200

  • Validations

    IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (Catalog No:111664, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

    IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (Catalog No:111664, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111664(IFT140 Antibody) at dilution of 1:300

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111664(IFT140 Antibody) at dilution of 1:300

    IP Result of anti-IFT140 (IP:Catalog No:111664, 4ug; Detection:Catalog No:111664 1:300) with rat testis tissue lysate 4400ug.

    IP Result of anti-IFT140 (IP:Catalog No:111664, 4ug; Detection:Catalog No:111664 1:300) with rat testis tissue lysate 4400ug.

  • Background
    IFT140 represents intraflagellar transport 140 homolog (Chlamydomonas)
  • References
    • Miller KA, Ah-Cann CJ, Welfare MF. Cauli: a mouse strain with an Ift140 mutation that results in a skeletal ciliopathy modelling Jeune syndrome. PLoS genetics. 9(8):e1003746. 2013.
    • Halbritter J, Bizet AA, Schmidts M. Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans. American journal of human genetics. 93(5):915-25. 2013.
    • Wei Q, Xu Q, Zhang Y. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes. Nature communications. 4:2750. 2013.
    • Yang Y, Ran J, Liu M. CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6. Cell research. 24(11):1342-53. 2014.
    • Martin CA, Ahmad I, Klingseisen A. Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nature genetics. 46(12):1283-92. 2014.
    • Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.
    • Ran J, Yang Y, Li D, Liu M, Zhou J. Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly. Scientific reports. 5:12917. 2015.
    • Siller SS, Burke MC, Li FQ, Takemaru K. Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells. Cell cycle (Georgetown, Tex.). 14(19):3163-72. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"