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IFNGR1 antibody

Cat.#: 111628

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Product Information

  • Product Name
    IFNGR1 antibody
  • Documents
  • Description
    IFNGR1 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 90 kDa
  • Tested applications
    ELISA, WB, IHC, IP, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD119 antibody; CDw119 antibody; FLJ45734 antibody; IFN gamma R1 antibody; IFN gamma receptor 1 antibody; IFNGR antibody; IFNGR1 antibody; interferon gamma receptor 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IFNGR1 recombinant protein (Accession Number: NM_000416). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111628(IFNGR1 antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111628(IFNGR1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111628(IFNGR1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111628(IFNGR1 antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-IFNGR1 (IP:Catalog No:111628, 4ug; Detection:Catalog No:111628 1:500) with HepG2 cells lysate 1800ug.

    IP Result of anti-IFNGR1 (IP:Catalog No:111628, 4ug; Detection:Catalog No:111628 1:500) with HepG2 cells lysate 1800ug.

    1X10^6 HepG2 cells were stained with .2ug IFNGR1 antibody (Catalog No:111628, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with .2ug IFNGR1 antibody (Catalog No:111628, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)
  • References
    • Rodriguez FJ, Giannini C, Asmann YW. Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. Journal of neuropathology and experimental neurology. 67(12):1194-204. 2008.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"