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Product Name
IARS2 antibody
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Description
IARS2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells, mouse lung tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 114 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10326 antibody; IARS2 antibody; IleRS antibody; Isoleucine tRNA ligase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IARS2 recombinant protein (Accession Number: BC010218). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111582(IARS2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:111582(IARS2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:111582(IARS2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using IARS2 antibody Catalog No:111582 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-IARS2 (IP:Catalog No:111582, 4ug; Detection:Catalog No:111582 1:800) with HeLa cells lysate 4000ug.
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Background
IARS2 represents isoleucyl-tRNA synthetase 2, mitochondrial
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