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Product Name
HUS1 antibody
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Description
HUS1 Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Checkpoint protein HUS1 antibody; hHUS1 antibody; HUS1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HUS1 recombinant protein (Accession Number: NM_004507). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: N/AIHC: 1:20-1:200
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111727(HUS1 antibody) at dilution of 1:0
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111727(HUS1 antibody) at dilution of 1:50 (under 10x lens)
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Background
Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].
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