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Product Name
HTRA1 antibody
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Description
HTRA1 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue. Positive IF detected in HepG2 cells. Positive IP detected in L02 cells. Positive WB detected in L02 cells, HepG2 cells, mouse brain tissue. Observed molecular weight by Western-blot: 35 kDa, 50 kD, 42 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARMD7 antibody; HtrA antibody; HtrA serine peptidase 1 antibody; HTRA1 antibody; L56 antibody; ORF480 antibody; PRSS11 antibody; Serine protease 11 antibody; Serine protease HTRA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002775). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111714(HTRA1 antibody) at dilution of 1:600
IP Result of anti-HTRA1 (IP:Catalog No:111714, 4ug; Detection:Catalog No:111714 1:500) with L02 cells lysate 1400ug.
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:111714(HTRA1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:111714(HTRA1 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of HepG2 cells using Catalog No:111714(HTRA1 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The antibody is specific to HTRA1.
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References
- Cai B, Zeng J, Lin Y. A frameshift mutation in HTRA1 expands CARASIL syndrome and peripheral small arterial disease to the Chinese population. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. 36(8):1387-91. 2015.
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