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Product Name
HSF1 antibody
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Description
HSF1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, K-562 cells, mouse heart tissue. Positive IP detected in Jurkat cells. Positive IHC detected in human lung cancer tissue, human testis tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 83kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Heat shOCk factor protein 1 antibody; HSF 1 antibody; HSF1 antibody; HSTF 1 antibody; HSTF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSF1 recombinant protein (Accession Number: NM_005526). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111556(HSF1 antibody) at dilution of 1:1000
IP Result of anti-HSF1 (IP:Catalog No:111556, 4ug; Detection:Catalog No:111556 1:1000) with Jurkat cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111556(HSF1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111556(HSF1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:111556(HSF1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
HSF1 represents heat shock transcription factor 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"