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Product Name
HSD17B4 antibody
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Description
HSD17B4 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human prostate cancer tissue, mouse heart tissue, mouse liver tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, mouse heart tissue, mouse liver tissue. Observed molecular weight by Western-blot: 79 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
17 beta HSD 4 antibody; D bifunctional protein antibody; DBP antibody; EDH17B4 antibody; HSD17B4 antibody; MFE 2 antibody; SDR8C1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSD17B4 recombinant protein (Accession Number: NM_000414). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111547(HSD17B4 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111547(HSD17B4 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111547(HSD17B4 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using HSD17B4 antibody Catalog No:111547 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HSD17B4 (IP:Catalog No:111547, 4ug; Detection:Catalog No:111547 1:500) with mouse brain tissue lysate 4000ug.
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Background
HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids.
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References
- Verheijden S, Bottelbergs A, Krysko O. Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation. Neurobiology of disease. 58:258-69. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"