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Product Name
HOP2 antibody
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Description
HOP2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, Jurkat cells. Positive IP detected in Jurkat cells. Positive IHC detected in human lung cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 24 kDa and 29 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
GT198 antibody; HOP2 antibody; HUMGT198A antibody; PSMC3 interacting protein antibody; PSMC3IP antibody; TBP 1 interacting protein antibody; TBPIP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HOP2 recombinant protein (Accession Number: NM_013290). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111523(PSMC3IP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111523(PSMC3IP antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using PSMC3IP antibody Catalog No:111523 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PSMC3IP (IP:Catalog No:111523, 4ug; Detection:Catalog No:111523 1:500) with Jurkat cells lysate 3200ug.
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Background
PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.
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References
- Cho NW, Dilley RL, Lampson MA, Greenberg RA. Interchromosomal homology searches drive directional ALT telomere movement and synapsis. Cell. 159(1):108-21. 2014.
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