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Product Name
HNRNPU antibody
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Description
HNRNPU Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells, mouse lung tissue. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hnRNP U antibody; HNRNPU antibody; HNRPU antibody; p120 antibody; pp120 antibody; SAF A antibody; SAFA antibody; Scaffold attachment factor A antibody; U21.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNRNPU recombinant protein (Accession Number: XM_017001117). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111512(HNRNPU antibody) at dilution of 1:500
IP Result of anti-HNRNPU (IP:Catalog No:111512, 4ug; Detection:Catalog No:111512 1:800) with HeLa cells lysate 2400ug.
Immunofluorescent analysis of HeLa cells using Catalog No:111512(HNRNPU Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HNRNPU represents heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
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References
- Suzuki H, Shibagaki Y, Hattori S, Matsuoka M. Nuclear TDP-43 causes neuronal toxicity by escaping from the inhibitory regulation by hnRNPs. Human molecular genetics. 24(6):1513-27. 2015.
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