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Product Name
HNRNPD antibody
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Description
HNRNPD Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells, mouse liver tissue. Positive IHC detected in human cervical cancer tissue, human placenta tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 38kd and 40kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AUF1 antibody; AUF1A antibody; hnRNP D0 antibody; HNRNPD antibody; hnRNPD0 antibody; HNRPD antibody; P37 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNRNPD recombinant protein (Accession Number: NM_002138). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111504(HNRNPD antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:111504(HNRNPD antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:111504(HNRNPD antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using HNRNPD antibody Catalog No:111504 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).
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References
- Neumann M, Valori CF, Ansorge O. Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. Acta neuropathologica. 124(5):705-16. 2012.
- Cooper-Knock J, Walsh MJ, Higginbottom A. Sequestration of multiple RNA recognition motif-containing proteins by C9orf72 repeat expansions. Brain : a journal of neurology. 137(Pt 7):2040-51. 2014.
- Chai F, Li HY, Wang W. Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection. Biochimica et biophysica acta. 1854(8):967-78. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"