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Product Name
HNF1B antibody
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Description
HNF1B Rabbit Polyclonal antibody. Positive WB detected in L02 cells, Jurkat cells. Positive IHC detected in human pancreas cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 58-60kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FJHN antibody; HNF 1 beta antibody; HNF 1B antibody; HNF1 homeobox B antibody; HNF1B antibody; HNF1beta antibody; HNF2 antibody; Homeoprotein LFB3 antibody; HPC11 antibody; LF B3 antibody; LFB3 antibody; MODY5 antibody; TCF 2 antibody; TCF2 antibody; Transcription factor 2 antibody; VHNF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNF1B recombinant protein (Accession Number: NM_000458). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111496(HNF1B antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111496(HNF1B antibody) at dilution of 1:50 (under 10x lens)
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Background
Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B
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References
- Shim JH, Lee HC, Han S, Kang HJ, Yu E, Lee SG. Hepatocyte nuclear factor 1β is a novel prognostic marker independent of the Milan criteria in transplantable hepatocellular carcinoma: a retrospective analysis based on tissue microarrays. Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society. 19(3):336-45. 2013.
- Min KW, Park MH, Hong SR. Clear cell carcinomas of the ovary: a multi-institutional study of 129 cases in Korea with prognostic significance of Emi1 and Galectin-3. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists. 32(1):3-14. 2013.
- Lai CR, Hsu CY, Chen YJ, Yen MS, Chao KC, Li AF. Ovarian cancers arising from endometriosis: a microenvironmental biomarker study including ER, HNF1ß, p53, PTEN, BAF250a, and COX-2. Journal of the Chinese Medical Association : JCMA. 76(11):629-34. 2013.
- Kim JY, Park do Y, Kim GH, Jeon TY, Lauwers GY. Does clear cell carcinoma of stomach exist? Clinicopathological and prognostic significance of clear cell changes in gastric adenocarcinomas. Histopathology. 65(1):90-9. 2014.
- Seth A, Ye J, Yu N. Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment. Development (Cambridge, England). 141(3):538-47. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"