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Product Name
HMOX1 antibody
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Description
HMOX1 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive IF detected in HT-1080 cells. Positive IP detected in HeLa cells. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells, HT-1080 cells, rat spleen tissue. Observed molecular weight by Western-blot: 28-33 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
bK286B10 antibody; heme oxygenase (decycling) 1 antibody; Heme oxygenase 1 antibody; HMOX1 antibody; HO 1 antibody; HO1 antibody; HSP32 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HMOX1 recombinant protein (Accession Number: NM_002133). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111491(HMOX1 antibody) at dilution of 1:600
IP Result of anti-HMOX1 (IP:Catalog No:111491, 3ug; Detection:Catalog No:111491 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111491(HMOX1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111491(HMOX1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HT-1080 cells using Catalog No:111491(HO-1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron .It has 3 isoforms:HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed(PMID:10194478 ).Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells(PMID:15451051 )and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin(PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa)(James R. Reed,Pharmacology ,535-568).
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References
- Song G, Cui Y, Zhong N, Han J. Proteomic characterisation of pancreatic islet beta-cells stimulated with pancreatic carcinoma cell conditioned medium. Journal of clinical pathology. 62(9):802-7. 2009.
- Chen N, Sun W, Deng X. Quantitative proteome analysis of HCC cell lines with different metastatic potentials by SILAC. Proteomics. 8(23-24):5108-18. 2008.
- Liu XH, Pan LL, Yang HB, Gong QH, Zhu YZ. Leonurine attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of reactive oxygen species and NF-κB pathways. European journal of pharmacology. 680(1-3):108-14. 2012.
- He HJ, Wang GY, Gao Y, Ling WH, Yu ZW, Jin TR. Curcumin attenuates Nrf2 signaling defect, oxidative stress in muscle and glucose intolerance in high fat diet-fed mice. World journal of diabetes. 3(5):94-104. 2012.
- Silva-Gomes S, Appelberg R, Larsen R, Soares MP, Gomes MS. Heme catabolism by heme oxygenase-1 confers host resistance to Mycobacterium infection. Infection and immunity. 81(7):2536-45. 2013.
- Pan LL, Liu XH, Shen YQ. Inhibition of NADPH oxidase 4-related signaling by sodium hydrosulfide attenuates myocardial fibrotic response. International journal of cardiology. 168(4):3770-8. 2013.
- Zhong L, Wang Y, Li W. Heme oxygenase-1 silencing increases the sensitivity of human osteosarcoma MG63 cells to arsenic trioxide. Molecular and cellular biochemistry. 392(1-2):135-44. 2014.
- Zhao F, Li K, Zhao L. Effect of Nrf2 on rat ovarian tissues against atrazine-induced anti-oxidative response. International journal of clinical and experimental pathology. 7(6):2780-9. 2014.
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