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Product Name
HMG20B antibody
- Documents
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Description
HMG20B Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 35-36 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BRAF25 antibody; BRAF35 antibody; BRCA2 assOCiated factor 35 antibody; FLJ26127 antibody; high mobility group 20B antibody; HMG box containing protein 20B antibody; HMG20B antibody; HMGX2 antibody; HMGXB2 antibody; PP7706 antibody; pp8857 antibody; SMARCE1 related protein antibody; SMARCE1r antibody; SOXL antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HMG20B recombinant protein (Accession Number: NM_006339). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111476(HMG20B antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111476(HMG20B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111476(HMG20B antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-HMG20B (IP:Catalog No:111476, 4ug; Detection:Catalog No:111476 1:800) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:111476(HMG20B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a eseential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].
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References
- Esteghamat F, van Dijk TB, Braun H. The DNA binding factor Hmg20b is a repressor of erythroid differentiation. Haematologica. 96(9):1252-60. 2011.
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