Product Name

HLA class I (HLA-A) antibody

Documents

Description

HLA class I (HLA-A) Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HEK-293 cells, HeLa cells. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human stomach cancer tissue. Observed molecular weight by Western-blot: 44 kDa

Tested applications

ELISA, WB, IHC, IF, IP, FC

Species reactivity

Human; other species not tested.

Alternative names

HLA A antibody; HLA class I antibody; HLA class I (HLA-A) antibody; HLAA antibody; HLA-A antibody; MHC class I antigen A*3 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of HLA class I (HLA-A) recombinant protein (Accession Number: NM_001242758). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

1X10^6 HepG2 cells were stained with 0.2ug HLA class I (HLA-A) antibody (Catalog No:111414, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:200.

HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111414(HLA class I (HLA-A) antibody) at dilution of 1:500

Immunofluorescent analysis of HepG2 cells using Catalog No:111414(HLA class I (HLA-A) Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-HLA class I (HLA-A) (IP:Catalog No:111414, 3ug; Detection:Catalog No:111414 1:500) with HepG2 cells lysate 1720ug.

Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:111414(HLA class I (HLA-A) antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:111414(HLA class I (HLA-A) antibody) at dilution of 1:100 (under 40x lens)

Background

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

References

Müller M, Agaimy A, Zenk J. The prognostic impact of human leukocyte antigen (HLA) class I antigen abnormalities in salivary gland cancer. A clinicopathological study of 288 cases. Histopathology. 62(6):847-59. 2013.
Dobbin ZC, Katre AA, Steg AD. Using heterogeneity of the patient-derived xenograft model to identify the chemoresistant population in ovarian cancer. Oncotarget. 5(18):8750-64. 2014.
de Carvalho JV, de Castro RO, da Silva EZ. Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection. PloS one. 9(11):e113691. 2014.

HLA class I (HLA-A) antibody

Product Information

Related Products / Services