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HLA class I (HLA-A) antibody

Cat.#: 107263

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Product Information

  • Product Name
    HLA class I (HLA-A) antibody
  • Documents
  • Description
    HLA class I (HLA-A) Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells, HeLa cells, Raji cells, U-937 cells. Positive IHC detected in human tonsillitis tissue, human heart tissue, human spleen tissue. Positive FC detected in HepG2 cells, HEK-293 cells. Observed molecular weight by Western-blot: 44 kDa
  • Tested applications
    ELISA, WB, FC, IHC
  • Species reactivity
    Human, Mouse, Rat, Pig; other species not tested.
  • Alternative names
    HLA A antibody; HLA class I antibody; HLA class I (HLA-A) antibody; HLAA antibody; HLA-A antibody; MHC class I antigen A*3 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of HLA class I (HLA-A) recombinant protein (Accession Number: NM_001242758). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107263(HLA class I (HLA-A) antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107263(HLA class I (HLA-A) antibody) at dilution of 1:500

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107263(HLA class I (HLA-A) Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107263(HLA class I (HLA-A) Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107263(HLA class I (HLA-A) Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107263(HLA class I (HLA-A) Antibody) at dilution of 1:200 (under 40x lens)

    1X10^6 HepG2 cells were stained with .2ug HLA class I (HLA-A) antibody (Catalog No:107263, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug HLA class I (HLA-A) antibody (Catalog No:107263, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
  • References
    • Zhao X, Sun Q, Tian H, Cong B, Jiang X, Peng C. Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China. Asian Pacific journal of cancer prevention : APJCP. 12(10):2741-5. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"