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Product Name
Histone-H3 antibody
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Description
Histone-H3 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells, HeLa cells. Positive IHC detected in human skin cancer tissue, human breast cancer tissue. Positive WB detected in MCF7 cells, HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue. Observed molecular weight by Western-blot: 15-17kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H3/n antibody; H3/o antibody; H3F2 antibody; H3FM antibody; HIST2H3A antibody; HIST2H3C antibody; HIST2H3D antibody; histone cluster 2 antibody; H3a antibody; Histone H3 antibody; Histone H3.2 antibody; Histone H3/m antibody; Histone H3/o antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Histone-H3 recombinant protein (Accession Number: BC015544). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117326(Histone-H3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:117326(Histone-H3 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using Catalog No:117326(Histone-H3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:117326(Histone-H3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
HIST2H3A,histone cluster 2, H3a.It is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. HIST2H3A is Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
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References
- Gong K, Xie J, Yi H, Li W. CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1 arrest, ROS-dependent apoptosis and differentiation in human leukaemia cells. The Biochemical journal. 443(3):735-46. 2012.
- Liu S, Li L, Zhang Y. The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. The Journal of biological chemistry. 287(36):30228-39. 2012.
- Tian D, Qiu Y, Zhan Y. Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability. Cardiovascular diabetology. 11:144. 2012.
- Tao H, Li H, Su Y. Histone methyltransferase G9a and H3K9 dimethylation inhibit the self-renewal of glioma cancer stem cells. Molecular and cellular biochemistry. 394(1-2):23-30. 2014.
- Chu G, Li Y, Dong X, Liu J, Zhao Y. Role of NSD1 in H2O2-induced GSTM3 suppression. Cellular signalling. 26(12):2757-64. 2014.
- Shi X, Chen G, Liu X. Scutellarein inhibits cancer cell metastasis in vitro and attenuates the development of fibrosarcoma in vivo. International journal of molecular medicine. 35(1):31-8. 2015.
- Xu LL, Zhu JF, Xu XL. Discovery and Modification of in Vivo Active Nrf2 Activators with 1,2,4-Oxadiazole Core: Hits Identification and Structure-Activity Relationship Study. Journal of medicinal chemistry. 58(14):5419-36. 2015.
- Li H, Liu Q, Wang Z. The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer. The Journal of biological chemistry. 290(37):22649-61. 2015.
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