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Product Name
Histone H1.2 antibody
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Description
Histone H1.2 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IF detected in Hela cells. Positive IP detected in HeLa cells. Positive WB detected in L02 cells, A375 cells, human testis tissue, Jurkat cells, MCF7 cells, mouse thymus tissue. Observed molecular weight by Western-blot: 32-33kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H1.2 antibody; H1F2 antibody; HIST1H1C antibody; histone cluster 1 antibody; H1c antibody; Histone H1.2 antibody; Histone H1d antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Histone H1.2 recombinant protein (Accession Number: BC002649). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111403(HIST1H1C antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:111403(HIST1H1C antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using HIST1H1C antibody Catalog No:111403 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-Histone H1.2 (IP:Catalog No:111403, 3ug; Detection:Catalog No:111403 1:500) with HeLa cells lysate 1600ug.
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Background
Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
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References
- Garg M, Perumalsamy LR, Shivashankar GV, Sarin A. The linker histone h1.2 is an intermediate in the apoptotic response to cytokine deprivation in T-effectors. International journal of cell biology. 2014:674753. 2014.
- Wu MY, Xie X, Xu ZK. PP2A inhibitors suppress migration and growth of PANC-1 pancreatic cancer cells through inhibition on the Wnt/β-catenin pathway by phosphorylation and degradation of β-catenin. Oncology reports. 32(2):513-22. 2014.
- Wang Y, Yue D, Xiao M. C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis. Journal of proteome research. 14(2):804-13. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"