Product Name

HGS antibody

Documents

Description

HGS Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 110-115 kDa

Tested applications

ELISA, WB, IP, IF, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

HGS antibody; HRS antibody; Protein pp110 antibody; ZFYVE8 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of HGS recombinant protein (Accession Number: NM_004712). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:500-1:5000

IHC: 1:50-1:500

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111314(HGS antibody) at dilution of 1:800

IP Result of anti-HGS (IP:Catalog No:111314, 5ug; Detection:Catalog No:111314 1:1000) with mouse brain tissue lysate 3000ug.

Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:111314(HGS Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:111314(HGS Antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of HeLa cells using Catalog No:111314(HGS Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

References

Holleman J, Marchese A. The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4. Molecular biology of the cell. 25(12):1892-904. 2014.

HGS antibody

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