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HEY1 antibody

Cat.#: 111308

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Product Information

  • Product Name
    HEY1 antibody
  • Documents
  • Description
    HEY1 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in DU 145 cells, mouse testis tissue. Positive IHC detected in human brain tissue, human kidney tissue. Observed molecular weight by Western-blot: 32-34 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BHLHb31 antibody; CHF 2 antibody; CHF2 antibody; HERP2 antibody; HESR 1 antibody; HESR1 antibody; HEY1 antibody; hHRT1 antibody; HRT 1 antibody; HRT1 antibody; OAF1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HEY1 recombinant protein (Accession Number: NM_012258). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    DU 145 cells were subjected to SDS PAGE followed by western blot with Catalog No:111308(HEY1 antibody) at dilution of 1:3000

    DU 145 cells were subjected to SDS PAGE followed by western blot with Catalog No:111308(HEY1 antibody) at dilution of 1:3000

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111308(HEY1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111308(HEY1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111308(HEY1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:111308(HEY1 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-HEY1 (IP:Catalog No:111308, 4ug; Detection:Catalog No:111308 1:500) with mouse kidney tissue lysate 4400ug.

    IP Result of anti-HEY1 (IP:Catalog No:111308, 4ug; Detection:Catalog No:111308 1:500) with mouse kidney tissue lysate 4400ug.

  • Background
    Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.
  • References
    • Jiang H, Cheng XW, Shi GP. Cathepsin K-mediated Notch1 activation contributes to neovascularization in response to hypoxia. Nature communications. 5:3838. 2014.
    • Xie H, Sun J, Chen Y, Zong M, Li S, Wang Y. 2B.06: EPIGALLOCATECHIN-3-GALLATE ATTENUATES URIC ACID-INDUCED INFLAMMATORY RESPONSES AND OXIDATIVE STRESS BY MODULATING NOTCH PATHWAY. Journal of hypertension. 33 Suppl 1:e23. 2015.
    • Kang H, Jeong JY, Song JY. Notch3-specific inhibition using siRNA knockdown or GSI sensitizes paclitaxel-resistant ovarian cancer cells. Molecular carcinogenesis. 2015.
    • Xie H, Sun J, Chen Y, Zong M, Li S, Wang Y. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative medicine and cellular longevity. 2015:214836. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"