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Product Name
HEXIM1 antibody
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Description
HEXIM1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human cervical cancer tissue, human placenta tissue. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells, human brain tissue, MCF7 cells, rat liver tissue. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 70, 54 kDa
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cardiac lineage protein 1 antibody; CLP1 antibody; EDG1 antibody; FLJ13562 antibody; HEXIM1 antibody; HIS1 antibody; MAQ1 antibody; Menage a quatre protein 1 antibody; Protein HEXIM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HEXIM1 recombinant protein (Accession Number: NM_006460). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111304(HEXIM1 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111304(HEXIM1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using HEXIM1 antibody Catalog No:111304 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HEXIM1 (IP:Catalog No:111304, 4ug; Detection:Catalog No:111304 1:500) with MCF-7 cells lysate 2000ug.
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Background
HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 70 and 54 kDa. Catalog#15676-1-AP recognises both 70 and 54 kDa band.
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References
- Galatioto J, Mascareno E, Siddiqui MA. CLP-1 associates with MyoD and HDAC to restore skeletal muscle cell regeneration. Journal of cell science. 123(Pt 21):3789-95. 2010.
- Mascareno E, Galatioto J, Rozenberg I. Cardiac lineage protein-1 (CLP-1) regulates cardiac remodeling via transcriptional modulation of diverse hypertrophic and fibrotic responses and angiotensin II-transforming growth factor β (TGF-β1) signaling axis. The Journal of biological chemistry. 287(16):13084-93. 2012.
- Neumann M, Valori CF, Ansorge O. Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. Acta neuropathologica. 124(5):705-16. 2012.
- Eilebrecht S, Benecke BJ, Benecke A. 7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb. RNA biology. 8(6):1084-93. 2012.
- Eilebrecht S, Le Douce V, Riclet R. HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters. Nucleic acids research. 42(8):4962-71. 2014.
- Espinoza-Derout J, Wagner M, Shahmiri K, Mascareno E, Chaqour B, Siddiqui MA. Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy. Cardiovascular research. 75(1):129-38. 2007.
- Espinoza-Derout J, Wagner M, Salciccioli L. Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1. Circulation research. 104(12):1347-54. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"